19 the initial loading to closer to a typical 5 to 40g loading per 1000 ml. C ase Study Wine Research Fig 7, 8, 9 & 10 by wine researchers ( 5) shows the worth of gradientsin L- LC, and of L- LC in unravelling difficult identification issues. This research led to the targets sensory properties being determined and tentative structural elucidation of new unknown oligomeric anthocyanins. The chromatogram on HPLC revealed an absence of standard baseline hump seen once the monomers etc were separated by L- LC. This highlights the value of doing a L- L chromatography sample polarity screening. L- LC helped these wine researchersto identify a new class of compounds ( oligomeric anthocyanin species), and to study their influence to the colour and sensory properties in wine. Case Study 7 HTPrep/ Combinatorial. In 2007 we custom designed the World's first Quattro HTPrep T for a Pharmaceutical Company in the USA. The research was presented at CCC2008 and published in the proceedings ( 6). Conclusions Liquid- Liquid Chromatography has matured into a valid science, which after almost 60 years deserves to be integrated into mainstream laboratory and process chromatography. L- LC been shown to compliment HPLC, with narrow range polarity cutting and by helping to find peaks co- eluting in HPLC. One beauty of L- LC is that the separation is based largely on defining on the polarities of targets, therefore classes of compounds can be separated which can then be optimised without sample loss. These narrow polarity range classes can finally be passed through a HPLC, assuming sample losses can be tolerated. If not, Sequential L- LC to L- LC with different solvents may be utilised. L- LC is a low-pressure technique ( typically 100 to 500 psi) thus it can use lower price ancillary equipment than HPLC. L- LC usage has the potential for considerable solvent cost and timesavings. References 1) Leitao G. G., El- Adji S. S., Melo, W. A., Leitao, S. G., Brown, L., J. Liq. Chromatogr. Relat. Technol. ( 2005) 28, 2041- 2051 2) Leitao G. G., Souza, P. A., Moraes, A. A., Brown, L., J. Liq. Chromatogr. Relat. Technol. ( 2005) 28, 2053- 2060 3) Comprehensive Analytical Chemistry XXXVIII, A Berthod ( Ed) ( 2002), Countercurrent Chromatography, Chapter 2, Berthod A, Brown L, Leitao G. G, Leitao. S. G. 4) Silva J. C. T., Jham G. N., Oliveira, R. D. L., Brown, L., J of Chrom A ( 2007), 1151, 203- 210 5) Vidal S., Hayasaka, Y., Meudec, E., Cheyner, V., Skouroumounis, G., J. Agric. Food Chem. ( 2004), 52, 713- 719 6) Wangenaar F. I., Hochlowski, J. E., Pan, J. Y., Tu, N. P., Searle, P. A, J Chrom A, 1216 ( 2009) 4154 - 4160. Figure 7 Figure 8 Figure 9 Figure 10 The NEW Thermo Scientific Chromatography Columns and Consumables Catalog The 2010 - 2012 catalog showcases the broadest collection of chromatography columns and consumables. Over 500 pages of high quality sample preparation, separation and analytical tools, including: . Extensive range of HPLC, GC and SPE products . Reagents and standards . Vials and closures www. thermo. com/ catalog Porvair Sciences 2009/ 2010 Catalogue now available For all your SPE and Microplate Needs To request a copy, please call or email on:- + 44 ( 0) 1372 824290 int. sales@ porvair- sciences. com Or to download a copy, visit: www. microplates. com

20November/ December 2009 Surface dissolution imaging gives insight into bioavailability Paraytec has announced an exciting new tool for surface dissolution imaging. The ActiPix SDI300 provides unique insights into processes taking place microns to millimetres from the surface enabling both erosion and dissolution phenomena to be visualised simultaneously. Initial results indicate that the SDI300 offers intriguing insights for researchers investigating bioavailability and a diverse range of substances including APIs, formulations, gels, liquids, stents and patches. For further information please contact Paraytec on + 44- 1904- 526270 or download the product Literature on http:// www. paraytec. com/ products/ actipix- dissolution- imager/ info@ paraytec. com or + 44 ( 0) 1904 526270 www. paraytec. com ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? Following the launch of the new Valco catalogue, Thames Restekis adding a number of new VICI products to their extensive range of chromatography consumables. These include: 360 micron high pressure fittingswhich allow direct connection of fused silica, PEEK, stainless or electroformed nickel tubing without the use of liners. A one- piece snap fitting nevertheless allows the ferrule to rotate freely as the nut tightens so that the tube doesn't twist. The compact size and fine threads create a leak- free connection that seals at pressures in excess of 20,000 psi and which can be easily formed with the available manual tool. Cheminert UHPLC injector, ideal for current analytical techniques requiring system pressures up to 20,000 psi, this comes in variable port versions, with bores as small as 100 µm. Cheminert Integrated Motor/ Valves for HPLC - this series is an integrated motor/ valve assembly, designed specifically to be built into an OEM system. Using the established injector design and a 24volt motor this is both compact and lightweight and needs only to be connected to the instrument's power supply. Dual Layer PEEK Tubingeliminates contamination issues by pigments used to color code the tubing, as the pigmented layer surrounds a separate but integrally bonded inner layer of natural PEEK. For more information on products or a copy of the catalogue, contact Thames Restek on 01494 563377 or go to www. thamesrestek. co. uk Thames Restek add new VICI Valco products to range Thermo Fisher Scientific ( USA) announce the introduction of a new program that invites scientists to trade in their single quadrupole or ion trap mass spectrometer - from any vendor - and upgrade to a Thermo Scientific linear ion trap technology. For a limited time only, the program will make the most advanced Thermo Scientific instruments accessible to single quadrupole and ion trap users. By trading- in their old single quadrupole or ion trap mass spectrometers, scientists can upgrade the analytical power of their laboratory with the Thermo Scientific LTQ XL or the LTQ Velos T the world's fastest and most sensitive LC- MS ion trap, or receive up to 30 percent off the price of a new Thermo Scientific linear ion trap. An additional benefit is the opportunity to further extend performance with an upgrade path to Thermo Scientific Orbitrap technology, offering ultra- high resolution, mass accuracy and increased dynamic range. For more information on this program please visit www. thermo. com/ tradeup Linear Ion Trap Trade- Up Program for Single Quadrupole and Ion Trap LC- MS Users