37 Grace Davison Discovery Sciences'is proud to announce that the revolutionary REVELERIS T flash chromatography system ( patents pending), introduced at Pittcon and Achema earlier this year, is the recipient of the coveted R& D 100 and IBO 2009 Industrial Design awards! For 47 years, R& D 100 awards have been given by R& D Magazine to the top 100 technological achievements of the year. Earlier award recipients have included lab- on- a- chip, the cardiac defibrillator, the fax machine, halogen lamps and liquid crystal displays. Grace will celebrate the innovation honours by joining a black tie event hosted by R& D magazine later this year. The REVELERIS T system is the direct result of Grace's expertise in material science, detector technology and continued investment in research and development. This is the fourth innovation distinction for the REVELERIS T system. It was a finalist in Laboratory Equipment Magazine's 2009 Readers' Choice Awards and a New & Notable mention at Pittcon. The REVELERIS T flash purification system utilizes proprietary RevealX T detection technology that integrates multiple detector signals that recognize ( detect) and collect components previously undetected, yielding more targeted samples with fewer impurities in less time, assisting medicinal chemists in more quickly developing promising drug candidates earlier in the development process. System capabilities are now expanded with the launch of the new release of version 1.1 RevealX Operating Software together with new REVELERIS T SRC C18 reversed phase cartridges. Find out more about the REVELERIS T system online at: www. discoverysciences. com/ Reveleris. New Flash Chromatography System Launches A New Generation Tool for Adsorption and Extraction of Volatile and Non- Volatile Compounds Applications include: Food & beverage Perfumes Aromas Forensics Environmental Biochemical Chemical For further information, a product brochure and free demonstration CD contact: Hichrom Ltd Cpds Vo ptc To Tp Compound VolatileandNon- V AdsorptionandExtra ANewGenerationT MonoTra el f fr pM olatil ctiono oolfo pTM pd: bge fs ssi s l l l pt o d @ k Applicationsinclude Food& beverag Perfume Aroma Forensic Environmenta Biochemica Chemica HichromLt) andfreedemonstrationCDc Forfurtherinformation, aproduc Email: sales@ hichrom. co. u Tel:+ 44( 0 he : ) 0 ontact brochur 118930366 Try any Restek column from Thames Restek before the end of January 2010 and save £ 150. Why not take this opportunity to look at the high performance Rxi ® columns. These columns are highly inert and exhibit ultra low bleed. Column to column reproducibility is guaranteed, with every new column performing exactly as the column it replaces. Every Rxi ® column undergoes stringent testing resulting in the most reliable columns available. To take advantage of this offer on all capillary GC columns quote " COL150" when placing any order. The technical team will be able to advise you on a Restek equivalent to your current column - call them on 01494 563377. A great reason to trial Restek Capillary GC Columns

Basics of Ion Exchange Chromatography IEX has been used for many years for analysis and purification of bio- molecules1. Its simple concept of charge induced reversible binding has several important advantages, two of which are: binding is fast and media show a high capacity. Also, compared with other chromatographic methods, such as hydrophobic interaction chromatography ( HIC) or mixed mode resins, method development is straightforward. The binding/ elution behaviour can be described by a simple " on/ off" mechanism. The molecules will bind to the chromatographic support at low ionic strength at a pH below ( for cation exchange) or above ( for anion exchange) its isoelectric point. Release will take place at increased ionic strength or by pH shift. In both cases, there is a distinct and narrow zone of pH / salt concentration, which determines whether there is binding or not. This also means that isocratic elution is not possible with IEX. Simple salt ( step) gradients are most commonly used for elution. Stationary phases are generally resistant to a wide range of pH. All these characteristics make the technique ideal for the two main process steps of capture and ( intermediate) purification. In addition final polishing steps can also be performed with IEX. The differences between these three steps are summarised in table 1. In a capture step the target compound is extracted and concentrated from the ( homogenised) fermentation broth where it is present in low concentrations. The main aim in this step is to concentrate the target compound, achieve complete recovery of the target compound and the removal of bulk impurities ( including protease etc.). In this step, high purity of the resulting concentrates is an advantage, but is not essential. During ( intermediate) purification the separation of the target compound from the main impurities is a key factor and purity aspects become more important. Even though IEX is a comparatively simple method, there are still several parameters to keep in mind when developing a cost effective large- scale production process. In the following section, some key factors having an impact on the efficiency of an IEX-process step are discussed. Capture step: In a capture step, the target compound is extracted and concentrated from the ( homogenised) fermentation broth, where it is present in comparatively low concentrations in the range from 1- 10 g/ L Facing the challenges in bio- pharmaceutical production: developments in ion exchange media to bring down cost of goods. Noriko Shoji1, Akiko Matsui1, Masakatsu Omote1, Naohiro Kuriyama1, Britta Blödorn ² , Daniel Kune ² , Charles A. White2 1YMC Co. Ltd., Ishikawa, Japan; 2YMC Europe GmbH, Dinslaken, Germany As the bio- pharmaceutical industry matures, terms like " cost of goods" are becoming more and more important. Up to now, strain optimisation for high productivity and upstream purification were the bottleneck for most bio- processes. However, with the progress made in recent years, titers in fermentation processes have increased significantly. Obviously, this increased volumetric productivity will help reducing the cost of goods, but it also has an impact on the downstream processing. Therefore, improved downstream processing media are required to process the increased product load in the same timeframe. Recently, new materials, based on fully synthetic polymer based matrices became available and show important advantages over traditional polysaccharide- derived media. In the following article the focus is on ion exchange chromatography ( IEX) as an important step in the biopharmaceutical process. 38November/ December 2009 Process stepImportant material characteristicsTypical Application Capture- Particle sizes between 45- 200 µm, sometimes higher- Harvest of fermentation - High dynamic binding capacity at high flow rates supernatants ( capturing) ( up to 1000 cm/ h and more) - Good flow characteristics. ( Intermediate) - Particle size ca. 30- 75 µm- Purification of material up to Purification- Low non- specific binding90+% purity, - Narrow particle size distribution- Reduction of endotoxins PolishingParticle size between < 10 - 30 µmPurification of up to 99+% Table 1 Media Characteristics for Typical Steps in Bioprocessing