4422November/ December 2009 Metrohm has developed a method for the analysis of Total Parental Nutrition ( TPN) solutions that are often administered to particularly vulnerable patients for example sick or newborn babies. TPN is a method of feeding that bypasses the gastrointestinal tract where the fluids are given into a vein to provide most of the necessary nutrients that the body needs. Typical electrolytes within a TPN solution include acetate, chloride, sodium, potassium, calcium and magnesium. Metrohm has developed methods for the analysis of both anion and cation analytes within TPN solutions using their Intelligent IC instruments. Total Parental Nutrition solutions represent a challenging sample matrix and deliver a mixture of fluid, electrolytes, calories, amino acids, vitamins, minerals and often fats into an infant's vein. Intelligent IC can be used to assay both the anion and cation constituents usually with analysis times of less than 20 minutes and the sample preparation is often no more complicated than a simple dilution. The intuitive nature of the MagIC Net controlling software means that the instrument can be run and a result obtained by a technician literally with a couple of mouse clicks. The electrolytes play a number of important roles within the body including regulation of hydration, blood pH and nerve & muscle function. It is vitally important that the constituents are carefully monitored as an ion imbalance can cause serious illness and in the event of a serious overdose can cause liver problems and has unfortunately resulted in infant death in a number of cases. Monitoring functionality and control charts within the software mean that the TPN assays can easily be profiled over a period of time and should values be outside of a strictly defined tolerance than the user is immediately notified through the software so that remedial measures may be instigated. For further information please contact enquiry@ metrohm. co. uk Analysis of Total Parental Nutrition Solutions Thermo Fisher Scientific announce a new application note entitled " Direct Analysis of Red Wine Using Ultra- Fast Chromatography." The application note explains the unique capabilities of ultra high pressure liquid chromatography ( U- HPLC) coupled to a Thermo Scientific Orbitrap mass spectrometer for use in the identification and quantitation of antioxidant constituents in red wine. The LTQ Orbitrap XL T hybrid mass spectrometer combined with Accela T U- HPLC delivers fast analysis times and accurate mass measurements, confirming the identity and structure of compounds in these complex mixtures. The application note is available for download at www. thermo. com/ redwine There is a growing interest among nutrition and health researchers in advanced liquid chromatography and mass spectrometry techniques. Red wine is a highly complex mixture and a rich source of beneficial antioxidants. It contains natural compounds that may, if consumed regularly and in moderation, have a positive impact on human health. Analysis of Antioxidants in Red Wine In complex matrices, however, as is the case with red wine, identification and quantitation of natural compounds is challenging. The application note demonstrates the potential of a direct analysis of red wine using the high resolving power of both the Accela and the LTQ Orbitrap XL. Researchers identified phenolic compounds responsible for most health benefits associated with red wine, such as quercetin, and they observed variable content across two different harvest years. Comparative analyses were performed using Thermo Scientific SIEVE 1.2 software. The features were filtered for statistical significance, with significant change defined as a minimum of two- fold concentration difference between the two harvest years. The results presented in the application note show statistically significant changes for individual compounds detected across two harvest years. The concentration differences for some compounds, particularly quercetin, were considerable. The LTQ Orbitrap XL delivers extremely high mass accuracy and resolution and is capable of multiple levels of fragmentation. The Accela U- HPLC affords fast analysis time while maintaining very high chromatographic resolution, which is important for complex matrices. The combination of the LTQ Orbitrap XL with Accela U- HPLC provides a robust and confident means of profiling complex mixtures as well as successful identification and advanced structural characterization of detected compounds without prior sample preparation. Advertise in our next issue... contact us on + 44 ( 0) 1727 855574, chromatographytoday@ ilmdesigns. co. uk or visit www. chromatographytoday. com ... we look forward to hearing from you

43 The presentations, stretched over three days covering a number of topical subjects including the new Metabolites in Safety Testing ( MIST) guidelines, dried blood spot analysis, large molecule analysis, incurred sample reproducibility ( ISR) and an update on current thinking on the subject of bioanalytical method validation from the FDA. Interspersed amongst these were also a number of scientific vendor presentations. There were two social networking events organised on the Tuesday and Wednesday evenings. On Tuesday, the evening meal and welcome drinks were served at Denbies vineyard, England's largest vineyard situated in Dorking, Surrey. On Wednesday evening the conference banquet and pre- dinner drinks were served in the University Season restaurant on campus. Mini- Exhibition As an integral part of the symposium, Tuesday saw 12 scientific vendor companies with tabletop displays of their latest product offerings. On Wednesday, it was the turn of the bioanalytical CROs which included ABS Labs, Cyprotex Discovery Ltd, Quality Assistance, York Bioanalytical, Quotient Bioresearch, Covance, BASI and HLS. The 18th International Reid Bioanalytical Forum Richard Houghton, Principal Scientist, Bioanalytical Sciences, Quotient Bioresearch Ltd. Clare Kingsley, Sector Manager, Bioanalytical Sciences, Quotient Bioresearch Ltd. The International Reid Bioanalytical Forum, initiated by Dr Eric Reid in 1975, is held every two years and offers an opportunity for industry, academia and analytical vendor organisations to come together and discuss the latest issues around bioanalysis. The Forum is run by the Forum Syndicate under the auspices of a sub- committee of the Chromatographic Society and draws on members from academia and industry. Its current members are Dr Howard Hill ( chairman and co- organiser), Dr Derek Stevenson, Forum organiser, Dr John Lough, Treasurer, Dr Stephen Westwood, Dr Robin Whelpton, and Dr Ian Wilson. The 18th International Reid Bioanalytical Forum was once again held on the Campus University of Surrey, from Monday 6th - Thursday 9th July 2009 with 120 registered delegates. Lectures were held in the Austin Pearce Building with the adjacent exhibition hall providing space for vendor stands and a poster exhibition. CompanyKey Products On DisplayContact BiotageSample preparation consumables www. biotage. com including SPE and SLE extraction cartridgesTel: + 46 18 56 59 00 GraceVisionHT UPLC columnswww. discoverysciences. com Tel: + 44 ( 0) 1480 32 4430 HichromHypersil Gold, Nucleodur and Zorbax RRHT www. hichrom. co. uk UPLC columns and chromatographic training servicesTel: + 44 ( 0) 118 930 3660 IDBSAdvanced data management solutionswww. idbs. com Tel: + 44( 0) 1483- 595000 Laboratory Electronic laboratory notebookswww. labnotes. com Data SolutionsTel: + 44 ( 0) 1904 686067 PresearchLaboratory solutions including separation equipment, www. presearch. co. uk automation equipment and suppliesTel: + 44 ( 0) 1256 365492 ShimadzuRecent Developments in Column and www. shimadzu. co. uk Instrument technologiesTel: + 44 ( 0) 8708 375209 Spark Online SPE extraction systems for sample cleanupwww. sparkholland. com Holland BVTel : + 31 591 631700 Supelco Ascentis T LC Columnswww. sigma- aldrich. com AnalyticalTel: 0800 717181 Thermo FisherHypersil Gold UPLC columnswww. thermo. com ScientificTel: + 44 ( 0) 1442 233 555 VarianHPLC systems and a wide range of column types and www. varianinc. com packing materials including Pursuit ® and Tel: + 44 ( 0) 1865 291 500 Polaris ® LC Columns WatersAcquity UPLC T hardware and columnswww. waters. com Tel: + 44 ( 0) 208 238 6100 Figure 1. University of Guildford - The venue for the 18th International Reid Bioanalytical Forum